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Two of these assumptions are highly relevant to stream fishes such as the brook trout ().

This potential problem can be overcome by restricting analyses to a single cohort or age-class.

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Second, because offspring emerge from discrete nests (redds) and show limited dispersal (Hunt and Brynildson ).

Further, typical sampling protocols for headwater salmonids involve starting in one location and working upstream until the desired sample size is achieved, which is likely to increase the probability of family over-representation.

In this paper, we examine the effect of sampling effort on the performance of the single-sample LD- = 1,440) of young-of-the-year (age-0) brook trout from each of three separate and currently isolated headwater habitat patches (the entire available habitat area within each creek) in Virginia, USA.

Previous work in one of the habitat patches demonstrated close kin associations within four months of emergence (Hudy et al.

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Family over-representation is likely to cause downward bias for estimates from the LD-).

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